Speaker: Adam Backer, Graduate student at ICME
Title: Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements
Abstract: In the past decade, a revolution has occurred in biological imaging. Using a conventional fluorescence microscope, it is now possible to image structures an order of magnitude smaller than the wavelength of light--thus achieving super-resolution. In this first part of my talk, I will briefly describe the basic experimental principles and computational methods that underlie single-molecule super-resolution microscopy. Next, I will present a novel image-processing technique for measuring the orientations and rotational dynamics of single fluorescent dye molecules over the course of a typical experiment. To demonstrate our approach, we use fluorescent dyes to obtain super-resolution images of stretched viral DNA. By leveraging our image data to obtain thousands of dye molecule orientation measurements, we develop a means of probing the nano-scale structure of individual DNA strands, while also characterizing dye-DNA interactions.
260-113 Pigot Hall (Language Corner)